Characterization of SARS-CoV-specific memory T cells from recovered individuals 4 years after infection.
Identifieur interne : 002B65 ( Main/Exploration ); précédent : 002B64; suivant : 002B66Characterization of SARS-CoV-specific memory T cells from recovered individuals 4 years after infection.
Auteurs : Yan-Ying Fan [République populaire de Chine] ; Zi-Tong Huang ; Li Li ; Man-Hui Wu ; Tao Yu ; Richard A. Koup ; Robert T. Bailer ; Chang-You WuSource :
- Archives of virology [ 1432-8798 ] ; 2009.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Conception de médicament, Cytométrie en flux, Facteur de nécrose tumorale alpha (biosynthèse), Facteurs temps, Femelle, Humains, Immunité cellulaire, Interféron gamma (biosynthèse), Interleukine-2 (biosynthèse), Jeune adulte, Lymphocytes T (immunologie), Lymphocytes T (virologie), Lymphocytes T CD4+ (immunologie), Lymphocytes T CD8+ (immunologie), Mâle, Mémoire immunologique, Production d'anticorps, Syndrome respiratoire aigu sévère (immunologie), Valeurs de référence, Virus du SRAS (immunologie), Études de suivi.
- MESH :
- biosynthèse : Facteur de nécrose tumorale alpha, Interféron gamma, Interleukine-2.
- immunologie : Lymphocytes T, Lymphocytes T CD4+, Lymphocytes T CD8+, Syndrome respiratoire aigu sévère, Virus du SRAS.
- virologie : Lymphocytes T.
- Adulte, Adulte d'âge moyen, Conception de médicament, Cytométrie en flux, Facteurs temps, Femelle, Humains, Immunité cellulaire, Jeune adulte, Mâle, Mémoire immunologique, Production d'anticorps, Valeurs de référence, Études de suivi.
English descriptors
- KwdEn :
- Adult, Antibody Formation, CD4-Positive T-Lymphocytes (immunology), CD8-Positive T-Lymphocytes (immunology), Drug Design, Female, Flow Cytometry, Follow-Up Studies, Humans, Immunity, Cellular, Immunologic Memory, Interferon-gamma (biosynthesis), Interleukin-2 (biosynthesis), Male, Middle Aged, Reference Values, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), T-Lymphocytes (immunology), T-Lymphocytes (virology), Time Factors, Tumor Necrosis Factor-alpha (biosynthesis), Young Adult.
- MESH :
- chemical , biosynthesis : Interferon-gamma, Interleukin-2, Tumor Necrosis Factor-alpha.
- immunology : CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes.
- virology : T-Lymphocytes.
- Adult, Antibody Formation, Drug Design, Female, Flow Cytometry, Follow-Up Studies, Humans, Immunity, Cellular, Immunologic Memory, Male, Middle Aged, Reference Values, Time Factors, Young Adult.
Abstract
SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular immune response from 21 SARS-recovered individuals who had been diagnosed with SARS in 2003 by using ELISA, CBA, ELISpot and multiparameter flow cytometry assays. Our results demonstrated that low levels of specific memory T cell responses to SARS-CoV S, M, E and N peptides were detected in a proportion of SARS-recovered patients, and IFN-gamma was the predominant cytokine produced by T cells after stimulation with peptides. Cytometry analysis indicated that the majority of memory CD8(+) T cells produced IFN-gamma, whereas memory CD4(+) T cells produced IFN-gamma, IL-2 or TNF-alpha. These results might provide valuable information on the cellular immune response in recovered SARS-CoV patients for the rational design of vaccines against SARS-CoV infection.
DOI: 10.1007/s00705-009-0409-6
PubMed: 19526193
Affiliations:
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Le document en format XML
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Koup</name></author><author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T" last="Bailer">Robert T. Bailer</name></author><author><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name></author></analytic><series><title level="j">Archives of virology</title><idno type="eISSN">1432-8798</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Antibody Formation</term><term>CD4-Positive T-Lymphocytes (immunology)</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>Drug Design</term><term>Female</term><term>Flow Cytometry</term><term>Follow-Up Studies</term><term>Humans</term><term>Immunity, Cellular</term><term>Immunologic Memory</term><term>Interferon-gamma (biosynthesis)</term><term>Interleukin-2 (biosynthesis)</term><term>Male</term><term>Middle Aged</term><term>Reference Values</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>T-Lymphocytes (immunology)</term><term>T-Lymphocytes (virology)</term><term>Time Factors</term><term>Tumor Necrosis Factor-alpha (biosynthesis)</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Conception de médicament</term><term>Cytométrie en flux</term><term>Facteur de nécrose tumorale alpha (biosynthèse)</term><term>Facteurs temps</term><term>Femelle</term><term>Humains</term><term>Immunité cellulaire</term><term>Interféron gamma (biosynthèse)</term><term>Interleukine-2 (biosynthèse)</term><term>Jeune adulte</term><term>Lymphocytes T (immunologie)</term><term>Lymphocytes T (virologie)</term><term>Lymphocytes T CD4+ (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Mâle</term><term>Mémoire immunologique</term><term>Production d'anticorps</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Valeurs de référence</term><term>Virus du SRAS (immunologie)</term><term>Études de suivi</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term><term>Interleukin-2</term><term>Tumor Necrosis Factor-alpha</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Facteur de nécrose tumorale alpha</term><term>Interféron gamma</term><term>Interleukine-2</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T</term><term>Lymphocytes T CD4+</term><term>Lymphocytes T CD8+</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD4-Positive T-Lymphocytes</term><term>CD8-Positive T-Lymphocytes</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Lymphocytes T</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Antibody Formation</term><term>Drug Design</term><term>Female</term><term>Flow Cytometry</term><term>Follow-Up Studies</term><term>Humans</term><term>Immunity, Cellular</term><term>Immunologic Memory</term><term>Male</term><term>Middle Aged</term><term>Reference Values</term><term>Time Factors</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Conception de médicament</term><term>Cytométrie en flux</term><term>Facteurs temps</term><term>Femelle</term><term>Humains</term><term>Immunité cellulaire</term><term>Jeune adulte</term><term>Mâle</term><term>Mémoire immunologique</term><term>Production d'anticorps</term><term>Valeurs de référence</term><term>Études de suivi</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular immune response from 21 SARS-recovered individuals who had been diagnosed with SARS in 2003 by using ELISA, CBA, ELISpot and multiparameter flow cytometry assays. Our results demonstrated that low levels of specific memory T cell responses to SARS-CoV S, M, E and N peptides were detected in a proportion of SARS-recovered patients, and IFN-gamma was the predominant cytokine produced by T cells after stimulation with peptides. Cytometry analysis indicated that the majority of memory CD8(+) T cells produced IFN-gamma, whereas memory CD4(+) T cells produced IFN-gamma, IL-2 or TNF-alpha. These results might provide valuable information on the cellular immune response in recovered SARS-CoV patients for the rational design of vaccines against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T" last="Bailer">Robert T. Bailer</name><name sortKey="Huang, Zi Tong" sort="Huang, Zi Tong" uniqKey="Huang Z" first="Zi-Tong" last="Huang">Zi-Tong Huang</name><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A" last="Koup">Richard A. Koup</name><name sortKey="Li, Li" sort="Li, Li" uniqKey="Li L" first="Li" last="Li">Li Li</name><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name><name sortKey="Wu, Man Hui" sort="Wu, Man Hui" uniqKey="Wu M" first="Man-Hui" last="Wu">Man-Hui Wu</name><name sortKey="Yu, Tao" sort="Yu, Tao" uniqKey="Yu T" first="Tao" last="Yu">Tao Yu</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Fan, Yan Ying" sort="Fan, Yan Ying" uniqKey="Fan Y" first="Yan-Ying" last="Fan">Yan-Ying Fan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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